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Fig. 4
osr2 and sox9a are coexpressed in the developing fin bud. Whole-mount in situ hybridization at 32 hpf shows that both osr2 (A) and sox9a (D) are expressed in the lateral plate in the region of the newly forming fin bud. osr2 (B) appears to be expressed throughout the fin bud, while sox9a (E) shows a more restricted expression. Transverse sections through the center of the fin bud show that osr2 is expressed uniformly throughout the fin bud mesenchyme (C), while sox9a-expressing chondrocytes are restricted to the center (F). Embryos in A, D are displayed from a dorsal view with anterior to the top. In B, E, arrowhead indicates fin bud with proximal to the left.