ZFIN Image: Sheehan-Rooney et al., 2013, Fig. S1

Image

Figure Caption

Fig. S1

Identification of a gata3 mutant zebrafish and expression of gata3. (A) The b1075 allele has a T>A point mutation (asterisk) in the 28^th bp of the fourth exon of gata3. The chromatograph shows a heterozygote. (B) The mutation generates a predicted C>S missense mutation in the conserved 2^nd Zinc Finger domain of Gata3 (G, Gallus gallus; H, Homo Sapien; M, Mus musculus; X, Xenopus laevis; D, Danio rerio, mut, b1075). (C) Schematic representation of the affected amino acid. (D-F) Embryos (E) injected with gata3 morpholino phenocopy (F) b1075 mutants. (G-J) Tissues disrupted in HDR express gata3. (G) Maxillary neural crest cells express gata3 (arrow). (H) Forming gill buds express gata3 by 72 hpf (arrows). (I) At 33 hpf, gata3 is expressed strongly in regions of the ear that will generate sensory cells (arrows). (J) Both the pronephric duct (pd) and Corpuscle of Stannius (cs) express gata3 at 33 hpf. Lateral views, anterior to the left. UIC=uninjected control.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.