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Fig. S2
Ethanol induces UPR. (A) Livers dissected from larvae exposed to 0 or 350 mM ethanol at 96-128 hpf were subject to standard PCR with xbp1 primers, and the products were run in 4% agarose gel to achieve efficient separation of spliced and unspliced bands. The image is inverted. The spliced and unspliced bands were quantified, added to make 100%. Fold changes were calculated by normalizing the percentage of spliced bands of ethanoltreated samples to that of untreated samples and plotted in Figure 2B. (B) Immunoblot of Bip and phosphorylated Eif2α of livers dissected from larvae exposed to 0 or 350 mM ethanol at 96-128 hpf. For each time point, protein was extracted from exactly 20 livers.