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Fig. S7
Generation and characterization of Tg (l-fabp:src-mC/CG) transgenic zebrafish. (A) Diagram of the l-fabp:src-mC/CG construct used in this study, containing Tol2 sequences, and the cmlc2:GFP expression cassette. (B) The src protein was expressed in the liver of wild-type and p53 mutant fish carrying the l-fabp:src-mC/CG transgene, as indicated by the cmlc2:GFP was expressed in the heart, as indicated by the green fluorescence. (C) (x 200) Immunohistochemical and (D) quantitative RT-PCR analysis of the expression of the src in hepatocytes from the 3, 5, 7, 9 and 11month old wild-type and p53 mutant fish overexpressing src and control l-fabp:GFP-mC transgenic fish. Scale bars: 50μm.