ZFIN Image: Zaghloul et al., 2010, Fig. 1

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Figure Caption

Fig. 1

Suppression of BBS4 produces specific defects. (A) Injection of MOs against individual bbs genes (bbs4 shown here) into zebrafish embryos produces gastrulation defects, including a short body axis, a widened and kinked notochord (*), and broadened somites (arrowhead), which can be rescued by WT human mRNA. Coinjections of mutant mRNAs produce a spectrum of defects. (B) Whereas WT RNA rescues the MO phenotypes (WT RNA), coinjection of hypomorphic mutations (N165H) partially rescues the phenotype; however, null mutations (D102G, N274H, and M472V in BBS4) do not rescue, and dominant-negative mutations (L327P) exacerbate the phenotype and produce defects by injection of RNA alone.

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Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA