ZFIN Image: Özhan et al., 2013, Fig. 5

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Figure Caption

Fig. 5

Lypd6 Interacts with Lrp6 and Partitions into Ordered Membrane Domains in Giant Plasma Membrane Vesicles

(A) spGFP-Lypd6 and spGFP-Lypd6ΔGPI-CD44 TMD coIP with Lrp6-HA, but not with Ldlr-HA, in HEK293T cells. spGFP-Lypd6ΔGPI does not coIP with Lrp6-HA or Ldlr-HA.

(B) spGFP-Lypd6 coIPs with Flag-xFz8 in HEK293T cells, and this binding is strongly enhanced by Wnt3a. Flag-xFz8 also binds to Lrp6-HA, which is enhanced by Wnt3a, but does not bind to Ldlr-HA. Lypd6 and Lrp6 still bind to Fz8 when expressed together.

(C–E) Partitioning of various GFP-tagged Lypd6 constructs in giant plasma membrane vesicles (GPMVs) derived from CHO cells. Fast DiI marks the disordered compartments.

See also Figure S4.

Acknowledgments

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Reprinted from Developmental Cell, 26(4), Özhan, G., Sezgin, E., Wehner, D., Pfister, A.S., Kühl, S.J., Kagermeier-Schenk, B., Kühl, M., Schwille, P., and Weidinger, G., Lypd6 Enhances Wnt/beta-Catenin Signaling by Promoting Lrp6 Phosphorylation in Raft Plasma Membrane Domains, 331-345, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell