ZFIN Image: Jao et al., 2013, Fig. 3

Image

Figure Caption

Fig. 3

Biallelic disruption of tyrosinase (tyr) by Cas9 generates mosaic pigmentation phenotypes. tyr gRNA (30 pg) and nls-zCas9-nls RNA (150 pg) were injected into wild-type embryos. (A–E) Lateral views of wild-type (A) and tyr-targeted embryos (B–E) at 2 dpf. tyr-targeted embryos showed different degrees of hypopigmentation, some of them were almost unpigmented (e.g., E). (F–K) Dorsal view of the wild-type (F and I) and tyr-targeted larvae (G, H, J, and K) at 7 dpf (F–H) and 14 dpf (I–K). Mosaic pigmentation defects in tyr-targeted larvae persisted to later stages. (L) T7EI assays showed high mutagenesis rates at the tyr target (93–97%) in six randomly selected tyr-targeted embryos. (Scale bars: 500 μm.)

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA