ZFIN Image: Lai et al., 2012, Fig. S8

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Figure Caption

Fig. S8 Knockdown of Atx-Lpar3 signaling activates β-catenin. (A-C<) Wild-type (Wt) embryos (A), and lpar3 (B) and atx (C) morphants were fixed at the 60-70% epiboly stage and immunostained with anti-β-catenin (green) and DAPI (red). Forty-five stacks ranging from 8.3 to 12.45 mm below the surface was calculated for colocalization (as indicated by white signal) using ImageJ software, merged and shown. Nuclear β-catenin signal was stronger in lpar3 and atx morphants than in Wt embryos. (D) Forty-five stacks in five embryos with respective treatments were quantified. groups denoted with different lettering refer to statistical significance (P<0.05).

Acknowledgments

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