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Fig. S7 Atx localizes in the shield during mid-epiboly stage. Atx was examined by immunofluorescence staining at the shield stage. (A) Atx was localized sporadically in blastomeres and concentrated in shield region (arrowheads) in control embryos. (B) The overall expression of Atx protein was notably reduced in atx morphants compared with that of control embryos. (C) When observed from the animal top view, Atx was found to be localized in shield blastomeres (arrowheads) in control embryos, but not those of atx morphants.
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