ZFIN Image: MacDonald et al., 2013, Fig. S2

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Figure Caption

Fig. S2 Diencephalic expression of gad1b is reduced in 48 hpf ascl1a morphants and in dlx1a/2a double morphants. Confocal images of fluorescent in situ hybridization in whole mount embryos for gad1b. (A) Control morpholino-injected embryos have normal telencephalic (Tel), prethalamic (Pth), and hypothalamic (Hy) expression of gad1b. (B) ascl1a morphants and (C) dlx1a/2a double morphants have decreased prethalamic and hypothalamic gad1b expression (asterisk). (D) Single dlx2a morphants do not show reduced gad1b expression. (E) dlx5a/dlx6a double morphants have decreased hypothalamic gad1b expression. (F) A diagram indicating the lateral field of view of confocal images in A–E. (G–H) Dorsal view of the forebrain in control morpholino injected fish and in ascl1a morphants (asterisk indicates a loss hypothalamic gad1b expression). (I) A diagram indicating the dorsal field of view of confocal images in G-H. Anterior is to the left. Scale bar=25 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 381(1), MacDonald, R.B., Pollack, J.N., Debiais-Thibaud, M., Heude, E., Talbot, J.C., and Ekker, M., The ascl1a and dlx genes have a regulatory role in the development of GABAergic interneurons in the zebrafish diencephalon, 276-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.