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Fig. S4
Dose-dependent rescue of miR-153 knockdown. (A) Single cell embryos were injected with a constant level of miR-153MO and increasing amounts (increments of 2 ng) of snap-25MOs. Embryo lysates from ~60 embryos in each group were prepared and SNAP-25 protein levels determined by western blotting. (B) Quantitation of westerns (n = 3) from A. The grey circle represents the amount of snap- 25MO (10 ng) used in co-injection rescue experiments.
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