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Fig. 3
miR-153 regulates endogenous snap-25a expression.
(A) Embryo lysates were prepared from either NIC embryos or embryos injected with miR-153, miR-153MO, mRNAs encoding snap-25a and snap-25b, morpholinos against snap-25, or combinations thereof, as indicated. Western blots were performed using antibodies against SNAP-25 and α–tubulin. (B) Quantification of SNAP-25 levels from the western blots (n = 3) shown in A. Significance was determined by a two-tailed Student’s t-test. Error bars show s.e.m.
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