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Fig. 2
Heat induced expression of EGFP and sdf1a in Tg(hsp70:sdf1a-nanos3, EGFP) embryos.
(A,B) Expression of EGFP was first observed at 30% epiboly in the embryos treated at 35.5°C, (C, D) 50% epiboly in the embryos treated at 34.5°C and (E, F) 2–5 somite in the embryos treated at 32.5°C. (G–N) EGFP expression was not detected by the 2 to 5 somite-stage in the embryos treated at 30.5 or 28.5°C. (O) The ubiquitous expression of sdf1a (dark blue) was quickly (less than 2 hours of staining) detected in heat-treated transgenic embryos started from late blastula stage. No obvious signal was seen in (P) non-heat-treated transgenic embryos, (Q) heat-treated wild-type embryos or (R) non-heat-treated wild-type embryos using the same staining condition. (S) No signal was detected when sdf1a sense riboprobe was used in heat-treated transgenic embryos as controls. Scale bar = 200 μm.