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Fig. S10
Knockdown of fn1 or tnc and overexpression of tnw results in cardia bifida in wild type embryos. Wild type embryos at the one-cell zygote stage were injected with 5 ng tnc-MO1 (B), 2.5 ng fn1-MO (C), 100 pg tnw mRNA (D), or a mixture of tnc-MO1, fn1-MO, and tnw mRNA (E), and incubated at 28.5°C. Un-injected embryos were used as a control (A). Embryos were harvested at the 22 ss and stained with cmlc2.
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