ZFIN Image: Omae et al., 2013, Fig. 4

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Figure Caption

Fig. 4

Identification of the circulation of gata1⁺ blood cells through V2^LP and V4^LP inter-organ vessels.

Time-lapse two-photon microscopy images of Tg(gata1:DsRed);Tg(fli1:egfp) larvae at 5 dpf (V1^LP, V2^LP, V4^LP) and at 6 dpf (V3^LP). No circulation of gata1⁺ blood cells was observed within V1^LP or V3^LP vessels during the 15 min. or 14 min. imaging periods, respectively. In contrast, the circulation of gata1⁺ blood cells was clearly observed both within V2^LP and V4^LP vessels during the 7 min. imaging period. The 3D rendered (3D) and/or Z-slices are shown. EGFP (green): fli1⁺ vessels; DsRed (magenta): gata1⁺ blood cells. The time-stamp (min: minute, sec: second) is indicated at the bottom right in each bottom panel. Scale bars: 30 μm.

Acknowledgments

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