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Fig. 5
Angiogenesis and increased expression of angiogenic factors in tumors from tsc2;p53 compound mutant zebrafish. (A,B) Arrowheads indicate visible blood vessels on tumors from abdomen (A) and eye (B) from p53zdf1/zdf1 zebrafish (n=8/16). (C,D) Arrowheads indicate large and tortuous blood vessels observed in abdomen (C) and eye (D) tumors from tsc2;p53 compound mutant (n=17/25) zebrafish. Scale bars: 1 mm. (E,H) Hif1-α staining, (F,J) Hif2-α staining and (G,K) Vegf-c staining in p53zdf1/zdf1 (E–G) and tsc2;p53 compound mutant (H–K) zebrafish. Scale bar: 100 μm.
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