ZFIN Image: Sorrell et al., 2013, Fig. 4

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Figure Caption

Fig. 4

Increased Wnt signaling prior to gastrulation promotes CM progenitor marker expression. ((A) and (B)) Fgf8a and ((C) and (D)) nkx2.5 expression at the 8s stage in control and wnt8.1 mRNA injected embryos. (E) Area and (F) fold difference in the areas of cells expressing fgf8a and nkx2.5. For analysis of fgf8a expression areas, control n=34 and wnt8.1 mRNA injected n=37. For analysis of nkx2.5 expression areas, control n=40 and wnt8.1 mRNA injected n=31. In contrast to what was observed in Tcf7l1 deficient embryos (Fig. 3), increased Wnt signaling prior to gastrulation from wnt8.1 mRNA injection expanded the expression of CM progenitor marker gene expression. All views are dorsal with anterior up. Arrows in (A)–(D) indicate the length of nkx2.5 and fgf8a expression. Asterisk indicates a statistically significant difference compared to controls.

Acknowledgments

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Reprinted from Developmental Biology, 380(2), Sorrell, M.R., Dohn, T.E., D'Aniello, E., and Waxman, J.S., Tcf7l1 proteins cell autonomously restrict cardiomyocyte and promote endothelial specification in zebrafish, 199-210, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.