|
Fig. 6
desmin and vinculin are not transcriptionally downregulated in mibta52b mutants.
Whole-mount in situ hybridization using desmin, vcla and vclb as mRNA probes. At 48 hpf, desmin is expressed at similar levels in the myogenic mesenchyme of the pectoral fins of siblings (n = 18) (A) and mibta52b mutants (n = 20) (B). At 5 dpf, the expression of desmin becomes stronger in mibta52b mutants (n = 19) (D) when compared with the sibling (n = 16) (C). Blebbistatin-treated embryos (n = 12) (F) show a similar level of desmin expression as control DMSO-treated (n = 10) (E). vcla is expressed strongly in the somites and has a faint expression in the sibling pectoral fins (n = 15) (G), the same expression pattern can be observed in the mibta52b mutant (n = 18) (H). vclb is expressed in the myogenic mesenchyme at 48 hpf in both siblings (n = 18) (I), (n = 5) (J) and mibta52b mutants (n = 19) (K), (n = 5) (L). At 5 dpf, the expression of vclb is poorly detected in the sibling embryos (n = 15) (M) and is still detected in mibta52b mutants (n = 16) (N). Transversal sections at the level of the pectoral fin of 48 hpf embryos are shown (J, L). Blebbistatin-treated embryos (n = 10) (P) show a similar level of vclb expression as control DMSO-treated (n = 12) (O).