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Fig. S7
Injection experiments of a mixture of mRNAs for BNip1 deletion mutants, related to Figure 6
(A) Western blotting of 7 hpf embryos injected with mRNAs for BNip1b deletion mutants using anti-BNip1b antibody. Stable protein expression of BNip1b-ΔSNARE (lane 2), BNip1b-Δcc (lane 3) and BNip1b-ΔTM (lane 4) was confirmed. (B) Confocal scanning of 7 hpf embryonic cells expressing GFP-tagged full-length BNip1b, BNip1b-ΔTM, BNip1b-Δcc and BNip1b-!SNARE. GFP-tagged full length BNip1b is localized to membrane organelle-like structures surrounding the nucleus, which probably correspond to the ER, whereas all other BNip1 deletion mutant proteins are uniformly observed in both cytoplasmic and nuclear regions. (C) Eight hpf embryos injected with BNip1b-Δcc mRNA plus standard MO, MO-Bax1 and MO-Bax2. (D) Histogram of the percentage of 8 hpf embryos classified as class I–IV in the injection experiments shown in (C). (E) Percentage of class I/II embryos. Green and black bars indicate the mean and standard deviation, respectively. **p<0.01. The number of injection experiments shown in (E) and p-values from t-tests are shown in Table S2.
Reprinted from Developmental Cell, 25(4), Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I., The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects, 374-387, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell