ZFIN Image: Pei et al., 2013, Fig. 2

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Figure Caption

Fig. 2 phox2b-deficient embryos show impaired differentiation of sympathetic neurons in the SCG.

(A–F) Lateral/oblique views of 4-dpf embryos after whole-mount ISH for th (A–C) and dbh (D–F) in control and phox2b–deficient embryos. Arrows indicate the SCG. Knockdown of phox2b by injection of a splice MO (4 ng) (MO^splice) inhibits the expression of th and dbh (B, E) which is rescued by coexpression of human PHOX2B mRNA (10 ng/μl) (C, F). Relative intensity levels of th (G) and dbh (H) expression in embryos injected with phox2b MOs that inhibit translation (MO^ATG) or splicing (MO^splice). Mismatched control MO (MO^mm) and PHOX2B mRNA-rescue (MO^splice/PHOX2B) are also shown. Data are presented as means ± SD (^***P<0.001; ^**P<0.01; n = 15 for each group).

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Acknowledgments

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