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Figure Caption
Fig. S1 miR-21 knockdown results in accumulation of miR-21 precursor and loss of mature miR-21. (A) Northern blot for short RNA molecules from control, miR-21-1 MO-injected, and miR-21-2 MO-injected embryos. Injection of miR-21-2 MO results in accumulation of the pre-miR-21 precursor (top band), which may be detected faintly in the miR-21-1 lane as well, but is absent from the control lane. (B) Injection of miR-21-2 MO causes a 90% reduction in the amount of mature miR-21 detectable by qPCR.
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