ZFIN Image: Helker et al., 2013, Fig. S3

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Figure Caption

Fig. S3 ECs of the CCV form tight and adherens junctions. (A-C) Fluorescent confocal images of immunostainings of the junctional proteins ZO-1 and Cdh5 at the indicated time points; lateral views. The developing vasculature is visualized by transgenic EGFP expression of Tg(fli1a:EGFP)^y1. (A-A′′) ECs of the CCV express the tight junction protein ZO-1 and the adherens junction protein Cdh5 at 26 hpf. (B-B′′) ECs of the CCV express the tight junction protein ZO-1 and the adherens junction protein Cdh5 at 32 hpf. (C-C′′) ECs of the CCV express the tight junction protein ZO-1 and the adherens junction protein Cdh5 at 48 hpf. Note that the epidermis shows nonspecific Cdh5 staining at 48 hpf.

Acknowledgments

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