|
Fig. 6 Disruption of dyx1c1 leads to pronephric brush border defects and loss of cilia dynein arms.
Ultrastructure of WT (3.5 dpf) pronephric duct showed dense brush border of apical microvilli extending from the epithelial cells and the presence of numerous cilia in the lumen (A). Apical microvilli were absent in dyx1c1 morphants (ATGMO+SPMO) at 3.5 dpf (B). Arrow in blue denotes missing brush border and blue arrowhead denotes cilia. Electron micrography of pronephric cilia axoneme in dyx1c1 morphant embryo at 3.5 dpf showed the absence of both ODA and IDA on the outer microtubules (D) whereas the dynein arms were present in WT embryos (C). Coinjection of dyx1c1 mRNA with MO rescued both ODA and IDA (E). Arrows in red indicate ODA and IDA in WT, morphant and rescued embryo. Scale bars in panels A and B indicate 1 μm and 2 μm respectively. Scale bars in panels C and D are 100 nm and 200 nm in E.