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Fig. 8 Overexpression of sfrp5 inhibits canonical Wnt signaling.
Clutches of 1- to 2-cell stage embryos obtained from an outcross of Tg(hs:mCherry,wnt2bb) heterozygotes with wild type fish were injected with either 100 pg eGFP or 100 pg sfrp5 and sorted based on expression of mCherry after heat shock. A-D) 48 hpf embryos were analyzed for liver formation by in situ hybridization with hhex and categorized as having an enlarged (A), normal (B), or small liver (C). We also observed some embryos without apparent hepatoblast (D) or in which the hepatoblast failed to coalesce into a single field (E). Square brackets indicate the size of the hepatoblast, the asterisk in (D) mislocalized hhex positive cells. F) Bar chart showing the percentages of each category per treatment. The total number of analyzed embryos per treatment is shown below each column.