Fig. 11 mRNA injection rescue studies indicate that tbx5a and tbx5b are not functionally redundant in pectoral fin bud outgrowth. A: Brightfield images of lateral pectoral fin region at 54 hours postfertilization (hpf). Dots outline the buds and are color-coded according to legend (see B). B: Percent of embryos exhibiting each phenotype (normal, partial or no pectoral fin outgrowth) at 54 hpf (tbx5a MO 82.5% n = 91; tbx5a MO+ tbx5a mRNA 78.6% n = 84; tbx5a MO+ tbx5b mRNA 89.5% n = 133; tbx5b MO 79.1% n = 220; tbx5b MO+ tbx5a mRNA n = 65, tbx5b MO+ tbx5b mRNA n = 69). Black or green dotted lines flank normal or truncated fin buds, respectively; bracket indicates area with missing fin.
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