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Fig. 5 Effect of feeder cells expressing zebrafish Lif, Fgf2 and Gdnf on FGSCs in 6-week cultures.
Photomicrographs showing two DsRed-positive FGSC colonies containing 100 or more cells (black arrows) under (A) bright field and (B) merged with UV (DsRed). (C) RT-PCR analysis of RNA isolated from 6-week FGSC cultures showing expression of germ cell specific maker genes, dazl, dnd, nanos3, vasa and ziwi. RT: reverse transcription; Scale bar = 20 μm.
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