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Fig. 4 Effect of feeder cells expressing zebrafish Lif, Fgf2 and Gdnf on 3-week FGSC cultures.
The presence of feeder cells expressing zebrafish Lif (OFC3L) significantly increased (A) FGSC colony number (p = 0.037) and (B) total FGSC number (p = 0.002) in 3-week cultures (* indicates a significant difference based on Student t test). (C) The presence of feeder cells expressing zebrafish Lif and Fgf2 (OFC3LF) or Lif and Gdnf (OFC3LG) did not significantly increase FGSC colony formation. (D) Addition of feeder cells expressing all 3 zebrafish factors (OFC3LF and OFC3LG) did significantly (p = 0.004) promote FGSC proliferation. (E) Photomicrograph showing a DsRed-positive (white arrow) FGSC colony containing about 28 cells that is growing on OFC3LF and OFC3LG. Data points not sharing a letter (A, B, C) are significantly different by Bonferroni–Dunn tests. Scale bar = 20 μm.