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Fig. 2 Production of ovarian-somatic feeder cell (OFC) lines.
(A) Diagram of the construct used to produce the Tg(gsdf:neo) fish. The plasmid contained a 3.8 kb gsdf promoter controlling expression of neo. (B) Neo (green) expressed in ovarian somatic cells, particularly the granulosa cells, of Tg(gsdf:neo). (C) The merger of (B) with the corresponding bright field photo. Photomicrograph showing 12 month-old (D) OFC1, (E) OFC3, (F) OFC5 and (G) OFC6 ovarian feeder cell cultures. Scale bar = 50 μm.
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