ZFIN Image: Rosen et al., 2013, Fig. 2

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Figure Caption

Fig. 2 ccm2l morphants exhibit heart and inflow tract defects. (A) Injection of MO e2i2 increases production of an endogenous alternatively spliced product (arrow) at the expense of the presumptive functional transcript (arrowhead). (B) Sequencing reveals that the alternatively spliced ccm2l mRNA, which is observed at increased levels in embryos injected with MO e2i2, incorporates intron 2, leading to an in-frame stop codon. (C and F) Uninjected embryos have compact heart chambers and narrow inflow tracts. (D,E,G,H) Embryos injected at the one-cell stage with 0.3 pMol MO e2i2 (D and G) or 1.0 pMol MO e4i4 (E and H) exhibit dilation of the atrium and inflow tract. Embryos injected with MO e2i2 often have a curved body axis. All images are taken from a lateral perspective with anterior to the left. a, atrium; i.t., inflow tract.

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Acknowledgments

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Reprinted from Developmental Biology, 376(1), Rosen, J.N., Sogah, V.M., Ye, L.Y., and Mably, J.D., Ccm2-like is required for cardiovascular development as a novel component of the Heg-CCM pathway, 74-85, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.