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Fig. 5
SHF progenitor cells fail to proliferate in the absence of Tbx1. A–D: Click-iT EdU labeling in 23ss Tg(nkx2.5:ZsYellow) vgo and control siblings. A,B: Flourescence microscopy images of EdU+ cells (red) in control (A) and vgo (B) embryos. 10× magnification, anterior up, dorsal right. Insets show flattened confocal images of ZsY+ cells comprising the cardiac cone. C,D: Composites of two confocal sections showing EdU+ cells (red) within the ZsYellow+ (green) SHF (white arrowheads). E: Graph depicting the average total numbers of EdU+, ZsYellow+ cells in confocal stacks of control (n=4) and vgo (n=4) embryos.
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