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Fig. 3
FIrst heart field development is unaffected in tbx1 mutants. A,B: Flattened confocal images of Tg(nkx2.5::ZsYellow) control (n=37; A) and vgo (n=14; B) embryos at 20.5hpf/23ss. C,D: Whole-mount in situ hybridization of vmhc at 20.5hpf/23ss in both control (n=16; C) and vgo (n=16; D) embryos. E,F: Flattened confocal images following immunofluorescence of cardiomyocyte nuclei comprising the linear heart tubes of 26 hpf control (E) and vgo (F) Tg(cmlc2:dsRed2-nuc) embryos. Dorsal view, anterior up in all images. G: Graph depicting the average number of CMs at 26 hpf in control and vgo embryos.
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