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Fig. S6
Notch signalling pathway overactivation leads to increased blastema marker expression and higher proliferation. (A-H) In situ hybridization of fins after a 12-hour heat-shock period (98-110 hpa). her6, her15, msxb and msxe expression is present in the distal region of the blastema in wild-type fins (A,C,E,G). Gene expression is stronger and expanded proximally in Tg(UAS:NICD) fish (B,D,F,H). (I) Double immunohistochemistry reveals co-labeling of Myc-NICD and BrdU in many cells (arrowheads). (J-K′) Immunohistochemistry of PCNA and aldh1a2 on 5 dpa fin sections. Double labelled cells (J9,K9) are restricted to the distal region of the wild-type fin (J, arrowhead) but is expanded proximally in Tg(UAS:NICD) fins at 5 dpa (K, arrowheads). (L,M) Immunohistochemistry against Osx of fins after a 12-hour heat-shock period (98-110 hpa). Scale bars: 10 μm in H,I,K,M. Dashed lines indicate the amputation plane.