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Fig. 1 Generation of Tg(fabp10a:def) transgenic fish.
(A) Diagram showing the fabp10a:def plasmid constructed in the pEGFP-C1. (B) Schematic drawing shows the genomic structure of the def gene from exon 4 to 6. Exon: blue box, intron: black line. Numerical number on the top indicates the cDNA nucleotide number beginning from the start codon ATG. Intron size was shown under the black line. Fw336 and Rv730 are the pair of primers used in PCR for screening of transgenic fish. (C) PCR products derived from the Tg-I and Tg-II transgenic fish. PCR products from the wildtype control fish (WT) and the Tg(fabp10a:def) plasmid were also shown. (D) Southern blotting analysis of the def transgene in Tg-I and Tg-II. DIG-labeled def cDNA was used as the probe.