ZFIN Image: Amali et al., 2013, Fig. S2

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Figure Caption

Fig. S2

Reduced expression of markers of primitive hematopoiesis gata1 and β embryonic globin (hbbe1) in hoxd4a morphants. WISH in control and morphant embryos at 26-28 hpf showing the expression of gata1 (A,C,E) and hbbe1 (B,D,F) in the ICM and PBI (white arrowheads). Normal expression of gata1 and hbbe1 (A,B) is severely reduced in hoxd4a morphants (C,D) and rescued by co-injection with capped mRNA for hoxd4a (E,F). All images are lateral views with anterior to the left. ctrl, embryos injected with a non-specific morpholino. MO, embryos injected with the anti-hoxd4a morpholino. hoxd4a mRNA, embryos simultaneously injected with the anti-hoxd4a MO plus capped mRNA for hoxd4a. Scale bars equal 100 μm. All images are at the same magnification.

Acknowledgments

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