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Fig. S4
Comparison of EGF-mediated Vesicular Trafficking of E-cad and EGFR-GFP
(Related to Figure 5)
(A-L) Co-immunofluorescence of E-cad (anti ECD; red) and EGFR-GFP (green) in WT (A-C), egf mRNA injected WT (D-F), MZspg (G-I), and egf mRNA injected MZspg (J-L) embryos at shield stage in fixed whole mounts. Merged channels reveal no colocalization between E-cad and EGFR positive endosomes in WT and MZspg embryos, as well as in WT and MZspg embryos overexpressing EGF. Insets in A-F show higher magnification. Animal pole views. Scale bar = 10 μm.
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Reprinted from Developmental Cell, 24(5), Song, S., Eckerle, S., Onichtchouk, D., Marrs, J.A., Nitschke, R., and Driever, W., Pou5f1-dependent EGF expression controls e-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements, 486-501, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell