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Fig. 3 MYCN promoted cell cycle progression and increased the ratios of myeloid cells and its precursors.
Single cell suspension from kidney of WT (A) and Tg(MYCN:HSE:EGFP) F2 generation (B) zebrafish was extracted at 60 dpf, treated with red blood cell lysis solution and stained with Propidium Iodide. Bars indicate the postion of modal DNA content peaks corresponding to the indicated G1 or S/G2 cell populations. It showed that MYCN increased the S/G2 ratio, histogram analysis in (C). In addition, elevated apoptosis was also detected in Tg samples as indicated by sub G1 peak (B). FACS analysed blood cells from kidney and spleen of WT (D, G) and Tg (E, H) fish at 60 dpf. Gated populations are as follows: erythrocytes, lymphocytes, myeloid cells, and blood cell precursors (morphologic feature was shown in D). All the detections repeated 3 times and analyzed in the two histograms (F, I) both indicated that myeloid cells and precursors were increased in Tg fish, correspondingly erythrocytes dramatically decreased, meeting the results of peripheral blood smear. **, P<0.01