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Fig. 6 Eaf1 and Eaf2 function as novel nuclear Wnt signaling components. (A) The domains in zebrafish β-catenin 1 and β-catenin 2 that interact with Eaf1 and Eaf2: +, interaction; –, no interaction. MID, the β-catenin center region that contains 12 Armadillo repeats. (B) Co-immunoprecipitation of Eaf1 or Eaf2 with full-length β-catenin 1 or β-catenin 2. (C) Eaf1 or Eaf2 did not interact with the N-terminus of β-catenin 1 or β-catenin 2. (D,E) Eaf1 or Eaf2 interacted with both the Armadillo repeats and the C-terminal transcriptional domain of β-catenin 1 (D) and β-catenin 2 (E). (F) The domains in Eaf1 and Eaf2 that interacted with β-catenin 1/2: N, exons 1-3 of Eaf1 or Eaf2; C, exons 4-6 of Eaf1 or Eaf2; +, interaction; –, no interaction. (G,H) β-catenin 1 (G) and β-catenin 2 (H) interacted with the N-terminus of Eaf1 or Eaf2. (I) β-catenin 1/2 did not interact with the C-terminus of Eaf1 or Eaf2. (J) Co-immunoprecipitation of Eaf1 or Eaf2 with full-length zebrafish c-Jun. WCL, whole cell lysate.