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Figure Caption
Fig. S8 Zebrafish Eaf1 or Eaf2 does not affect the protein level of either β-catenin 1 or β-catenin 2. (A) Western blot analysis for total β-catenin and active β-catenin protein (Ac-catenin) in embryos injected with STD-MO (lane 1), Eaf1- MO1 (lane 2), Eaf2-MO1 (lane 3), Eaf1-MO3 (lane 4) or Eaf2-MO3 (lane 5) (8 ng/per embryo). (B) The co-transfection of increased amounts of eaf1 or eaf2 together with β-catenin 1 and β-catenin 2 did not change the protein level of β-catenin 1 and β-catenin 2. (C) Western-blot analysis confirmed ectopic expression of zebrafish Eaf1 and Eaf2.
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