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Fig. S4 Ectopic expression of zebrafish Eaf1 and Eaf2 increases the expression of sqt and gsc. (A) At the gastrula stage, dorsal marker genes sqt (a-c) and gsc (d-f) displayed expanded expression, particularly in the ventral domain. qRTPCR was used to measure expression of sqt (g) and gsc (h) in these embryos. (B) In embryos injected with dn-Tcf mRNA (b) or frzb mRNA (c), the expression of gsc expanded ventral-laterally at the gastrula stage, compared with embryos injected with GFP mRNA (a). Dorsal views.
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