Fig. S2

Fig. S2 Membrane-bound cargo accumulated in jip3^nl7 axon terminals due to failed retrograde transport. (A,B) ssNPY-mCherry (red) accumulated in mutant (arrowheads), but not wildtype axon terminals at 5 dpf. Asterisk indicates portion of the nerve occluded by a pigment cell. The pLL axons were visualized by expression of the neurod:EGFP transgene. (C–F) Representative still images from Videos S2 and S3 and kymographs from transport analysis in 5 dpf larvae. (G) Analysis of anterograde and retrograde ssNPY-mCherry particle movements at 2 dpf revealed a decrease in anterograde and retrograde cargo movement (ANOVA; **-p<0.005). (H, I) Other transport parameters, including distance moved in individual movement bouts and movement velocity, were unchanged in jip3^nl7. Scale bars = 10 μm. Number of embryos analyzed is indicated on each bar of the graph.