|
Fig. 2 Lgl2 depletion disrupts KV lumen and cilia formation. (A,B) Ciliated KV cells were labeled by acetylated tubulin immunostaining (red) and GFP expression (green) in Tg(sox17:GFP) embryos injected with control (A) or Lgl2 (B) MO. Scale bars: 20 μm. (C-E) KV cilia number (C), KV cilia length (D) and KV lumen size (E) were significantly reduced in Lgl2 MO embryos compared with MO control and uninjected controls. lgl2 mRNA partially rescued each of these defects. (F) The number of KV cells was similar among control and Lgl2 MO embryos in Tg(dusp6:memGFP) and Tg(sox17:GFP) transgenic lines. Error bars indicate s.d. n, number of embryos analyzed. *P<0.05.