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Figure Caption
Fig. S2 Depletion of Lgl2 reduced KV lumen volume, but did not alter localization of apical epithelial markers. (A) Analysis of KV lumen volume at the 8-somite stage in live Tg(dusp6:memGFP) embryos. Error bars represent s.d. n, number of embryos analyzed. *P<0.05. (B,C) Fluorescence staining of apical markers in Tg(dusp6:memGFP) embryos injected with control MO or Lgl2 MO. ZO-1 (B) and F-Actin (C) were localized at apical membranes lining the KV lumen in both control and Lgl2-depleted embryos. Scale bars: 20 μm.
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