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Figure Caption
Fig. S3 DFC clustering was disrupted in a subset of Lgl2 knockdown embryos. (A-B′) RNA in situ hybridization of sox17 expression was used to analyze DFCs (arrows) at the 90% epiboly stage. A′ and B′ are magnified views of DFCs in A and B, respectively. (C) DFCs were tightly clustered in most control embryos (A,A′C), whereas DFCs were dispersed in 23% of Lgl2 MO embryos (B,B′,C). n, number of embryos analyzed.
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