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Fig. S6
HOXC9 overexpression rescues the defects in parachordal lymphangioplast (PL) formation in Stab2 morphants at 72 hpf.
(A) Overall morphology of 72 hpf zebrafish embryo after control morpholino injection. Red box shows region displayed in (B–D). (B) Normal formation of the PLs (arrows) in 72 hpf tg(fli1:EGFP) zebrafish embryo after injection of 4 ng control morpholino. (C) Silencing of Stab2 expression using 4 ng splice-blocking morpholino disrupted the formation of the PLs (asterisks) in 72 hpf tg(fli1:EGFP) zebrafish embryo. (D) Injection of HOXC9 mRNA (50 pg) rescued the Stab2 loss-of-function phenotype in 72 hpf tg(fli1:EGFP) zebrafish embryo. (E) Quantification of 72 hpf tg(fli1:EGFP) fish embryos showing a disturbed PL formation including rescue experiments using HOXC9 mRNA (50 pg). Embryos were divided in three groups depending on the PL appearance being completely absent, partially formed or completely present. Black scale bar: 500 μm. White scale bar: 50 μm.