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Fig. S5
HOXC9 overexpression rescues defects in parachordal lymphangioplast (PL) formation in Stab2 and Stab1 morphants.
(A–E′) Whole mount antibody staining against GFP in 48 hpf (A–E) and 72 hpf (A′–E′) tg(fli1:EGFP) zebrafish embryos injected with the indicated morpholinos. (A,A2) Normal formation of the PLs (arrows) in 48 hpf (A) and 72 hpf (A′) tg(fli1:EGFP) zebrafish embryos after injection of 4 ng control morpholino. (B,B′) Silencing of Stab2 expression using 4 ng Stab2-Ex2-Mo disrupted formation of the PLs (asterisks) in 48 hpf (B) and 72 hpf (B′) tg(fli1:EGFP) zebrafish embryos. (C,C′) Injection of HOXC9 mRNA (50 pg) rescued the Stab2 loss-of-function phenotype in 48 hpf (C) and 72 hpf (C′) tg(fli1:EGFP) zebrafish embryos. (D,D′) Silencing of Stab1 expression using 4 ng Stab1-Ex3-Mo disrupted formation of the PLs (asterisks) in 48 hpf (D) and 72 hpf (D′) tg(fli1:EGFP) zebrafish embryos. (E,E′) Injection of HOXC9 mRNA (50 pg) rescued the Stab1 loss-of-function phenotype in 48 hpf (E) and 72 hpf (E′) tg(fli1:EGFP) zebrafish embryos. Black scale bar: 500 μm.