|
Fig. S4 Silencing of Stab2 expression using an ATG-morpholino in zebrafish inhibits assembly of parachordal lymphangioplasts (PLs). (A;D) Overall morphology of 48 hpf (A) and 72 hpf (D) zebrafish embryos after injection of control morpholino. Red box shows region displayed below in (B,C,E,F). (B,E) Normal formation of the PLs (arrows) in 48 hpf (B) and 72 hpf (E) tg(fli1:EGFP) zebrafish embryos after injection of 4 ng control morpholino. (C,F) Silencing of Stab2 expression using 2 ng translational-blocking morpholino disrupted the formation of the PLs (asterisks) in 48 hpf (C) and 72 hpf (F) tg(fli1:EGFP) zebrafish embryos. (G,H) Quantification of 48 hpf (G) and 72 hpf (H) tg(fli1:EGFP) zebrafish embryos showing a disturbed PL formation. Embryos were divided in three groups depending on the PL appearance being completely absent, partially formed or completely present. Black scale bar: 500 μm. White scale bar: 50 μm.