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Fig. 1 Structure, endoderm-inducing activity and subcellular localization of wild-type cas and a deletion variant. (A) D-Cas contains a deletion of the conserved EFDQYLN peptide located in the C-terminal region (red in the wild type) that is likely to act as an activation domain. cas and D-cas RNAs were used for injection. (B-F) Zebrafish embryos were left uninjected (B) or injected with the RNA indicated bottom right and probed by in situ hybridization for sox17 (B-D) or by immunohistochemistry with anti-myc antibody (E,F). The inset in F indicates the position of myc-positive nuclei (arrows). (G) Immunoprecipitation (IP) for myc-D-Cas and myc-Cas. The myc-Cas and myc-D-Cas proteins are detected at 45 kDa.