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Fig. s3 Embryos injected with a five-base mismatch morpholino show unaltered expression of neural markers. The specificity of the akt1 morpholinos was confirmed by injection with a morpholino with a five-base mismatch relative to MO2. Injection of this morpholino resulted in unaltered expression of neural markers showing by in situ hybridization (A) and quantitatively confirmed by qPCR (B).
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