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Fig. 4 Ectopic notum1a expression or SU5402 treatments perturb axis elongation. (A-M) Wild-type (A-C,F,K), notum1a-overexpressing (D,E,G,L) and SU5402-treated (H-J,M) zebrafish embryos. (C) notum1a is transcribed in the posterior DM. (D) Ectopic notum1a gives rise to a shortened axis and malformed tailbud. (E) Half of these embryos develop severely misdirected body axes. Spatial expression of tbx6 (F-H) and neurogenin1 (K-M) remains relatively normal despite notum1a overexpression (G,L) or SU5402 treatment (H,M). (I,J) SU5402 treatment gives rise to a mild axis elongation defect. (N) qPCR of dissected tailbuds after notum1a overexpression reveals reductions in transcription of dkk1 and tbx6. Student′s unpaired t-test. *P≤0.05. n=4. Error bars represent s.e.m.