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Fig. 4 Hedgehog or Igf pathway activation increases cardiomyocyte proliferation during regeneration in zebrafish. (A) Treatment with SAG (2.5 μM) or NBI-31772 (10 μM) from 6 to 7 dpa increased cardiomyocyte proliferation. Mef2, red; PCNA, green. Brackets indicate injury site. Insets: High magnification of the boxed areas. Arrowheads indicate proliferating cardiomyocytes. Scale bar: 50 μm. (B) Quantification of cardiomyocyte proliferation following treatment with SAG (2.5 μM) after resection (left) or ablation (right) injury models. Fish were treated from 6 to 7 dpa. n=9-15, mean±s.e.m. *P<0.01, Student′s t-test. (C) Quantification of cardiomyocyte proliferation following treatment with NBI-31772 after resection (10 μM) or ablation (5 μM) injury models. n=11-17, mean±s.e.m. *P<0.05, Student′s t-test.